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SNP detection

SNP (Single Nucleotide Polymorphism)

Genetic variation showing a difference in one base (A, T, G, C) in the DNA sequence is called Single Nucleotide polymorphism (SNP).
When an allele is present with a frequency of 5% or more, it is called common polymorphism, and when it is 1 to 5%, it is classified as rare polymorphism.

SNP analysis using specific bidirectional
kidney technology (SBDE)

By using the unique bidirectional stretching technology, the original technology of Heim Biotek, it is possible to accurately distinguish homozygote and heterozygote simultaneously with one experiment.

Existing method
(eg. Allele Specific-PCR)

snp_img

HB SBDE-PCR

snp_img

Sensitivity improvement using
Switching-SBDE PCR technology

The cross-reaction and low yield, which are the disadvantages of the existing AS-PCR, have been compensated,
and even a small amount of sample has high specificity and sensitivity.

Advantage of HB Switching AS-PCR technology based on T790M detection

[General AS-PCR Contrast]

Switching PCR
sens_gray_box_img
Condition
95 ℃ 15 min
95 ℃ 15 sec 20 Cycle
65 ℃ 30 sec
72 ℃ 30 sec
95 ℃ 15 sec 40 Cycle
71 ℃ 30 sec
72 ℃ 30 sec
Switching AS-PCR result
>1/8% & 1/10% mutant typing available at 95% level
General PCR
sens_gray_box_img
Condition
95 ℃ 15 min
95 ℃ 15 sec 60 Cycle
71 ℃ 30 sec
72 ℃ 30 sec
General AS-PCR results
>1/8% & 1/10% mutant typing impossible at 95% level
logoImg
Heim Biotek Co., Ltd. : Pangyo Silicon Park A-201 35, Pangyo-ro 255beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea
Company Registration Number : 135-86-48279
Tel : +82-1522-7026
Fax : +82-031-548-2135
E-mail : info@heimbiotek.com
Copyright ⓒ 2020 Heim Biotek All rights reserved.

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